What type of tissue are you looking at? Why don’t you just stain with toluidine blue or safranin O. Both of these stain proteoglycan, areas of cartilage damage will show loss of staining. There are also pathology parameters such as fibrillation, loss of chondrocytes and chondrocyte cloning that are characteristic of cartilage damage. If you feel you need to use a IHC stain, you could try collagen II. Articular cartilage should stain positive for collagen II. But I would initally try the special stains.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
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phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Walters, Katherine S
Sent: Thursday, September 06, 2007 10:46 AM
To: histonet@pathology.swmed.edu
Subject: [Histonet] damaged cartilage
Does anyone know of a good antibody for damaged cartilage? I found one very expensive one in Canada, but it is cost prohibitive.
Thanks,
Kathy Walters
Katherine Walters
Histology Director
Central Microscopy Research Facility
University of Iowa
85 Eckstein Medical Research Building
Iowa City, Iowa 52242-1101
phone: (319) 335-8142
fax: (319) 384-4469
katherine-walters@uiowa.edu
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