RE: [Histonet] Xylene

From:"Kemlo Rogerson"

Can somebody tell me if there are any effects on tissue if there is
prolonged processing time in xylene? 

Jason McGough HT(ASCP)
Clinical Laboratory of the Black Hills
Account Representative - Anatomic Pathology 
2805 5th Street
Rapid City, SD 57701

Classically it is said to harden, plus more lipids could be removed.
Personally I'm equivocal about that thought; if properly fixed prior to
processing I would have thought the hardening effects of xylene were
much less than that of the coagulant fixative ethanol. If you do have
hard tissue then there is a restorative fluid one can use which I think
has oil of cedarwood in it but I don't know the formula off hand. I know
it works cos when I was a pup I used it sometimes.

Kemlo Rogerson
Pathology Manager
DD   01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;

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