Lots of mounting media and the smallest coverslip possible, that way you have less chance for bubbles.  When we mounted whole rat corneas we placed the mounting media on top of the section and carefully placed the coverslip over the top of the specimen.  Then we used some nuts from the hardware store and placed them on the coverglass to flatten out the specimen and let dry overnight at room temp we found that heating the slides caused more air bubbles.

Liz 

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
liz@premierlab.com
www.premierlab.com
 
Ship to Address:
 
Premier Laboratory, LLC
University of Colorado at Boulder
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Boulder, CO 80309

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of alonso.martinezcanabal@utoronto.ca
Sent: Friday, September 14, 2007 11:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Golgi-Cox issue

   Hi,

         I am working with Golgi-Cox sections, at 120 microns. I everything is very good, except that I cannot mount the sections very well, apparently the tickness is causing troubles and the sections get dry-like some days after.

  Any sugiestions to mount with permount the tick sections?

   Thank Yoy very much



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