RE: [Histonet] Gloves for Microtomy

From:"Tony Henwood"

It is not impossible to change film with gloves on.
In our Electron Microscopy Unit we have to load 5x4 film (single sheets)
in the dark wearing cotton gloves (stops greese from contaminating the
column - one hopes!).
It takes practice.
I only wear gloves when I have to cut thick paraffin sections for RNA
and DNA extractions, which we seem to be on the  increase.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Walzer
Sent: Monday, 10 September 2007 5:40 PM
To: Bill;
Subject: RE: [Histonet] Gloves for Microtomy

Gloves have NEVER been required to cut paraffin blocks. Gloves would
more than likely be the cause of accidents as they would get in the way
cutting. Has anyone ever tried to change the film in a camera with
gloves on? Doesn't work...

-----Original Message-----
[]On Behalf Of Bill
Sent: Friday, September 07, 2007 3:14 PM
Subject: Re: [Histonet] Gloves for Microtomy

I have formally placed lab inspectors in the same category as 
lawyers, regulators and politicians.


At 12:57 PM -0500 9/7/07, Dawson, Glen wrote:
>I have a rogue JCAHO inspector currently on-site that is demanding
>that all of the histotechs cut paraffin blocks with gloves on.  She 
>has made up many other rules of her own but most were so outlandish 
>that they were easily discounted.

Bill Blank (Celtic studies and numismatics)
OBOD's Message board:

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