[Histonet] toluidine staining faded!

From:"Moran Elishmereni"

Hello histonetters,

I stained some murine skin sections for toluidine blue, and mast cells were
very easy to detect, however, after a few days the staining seemed to have
faded. Following the tol blue staining, I briefly put the sections in
alcohol (95%, 100%, 100%), then in xylene substitute, then air dryed and
mounted with mounting media. What did I do wrong? I am very new
at histology, so forgive my simple question...


Moran Elishmereni

Department of Pharmacology and Experimental Therapeutics
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
POB 12065
Jerusalem 91120, ISRAEL
Tel: 972-2-675-8746
Fax: 972-2-675-8144
Email: moran.elish@gmail.com
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