Can anyone recommend a protocol for frozen section technique - to cut
Brain tumor biopsies.
The usual ice-crystal artifact sometimes renders the Frozen Sections
unreadable for intra-operative diagnosis.
We use a Leica UV1850 cryostat set at -21*
and cut our FS at 8 microns
rapid stain H/E
The pathologist here tends to believe it is our technique that produces
fast freezing with spray vs slow freezing??????????
We have tried a lot of methods to eliminate this problem
HELP!!!!!!!!!!! I know someone out there has a remedy for the headache
this has created!!!!
Thank you all in advance!!!
I am glad I can pose questions to others who might be dealing with the
issues I deal with too!
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