[Histonet] Freezing mouse testes for frozen sections

From:Sarah Clatterbuck Soper

Hi all,

I've started attempting to section unfixed frozen mouse testes in order 
to placate a specific antibody we have to use.  I am new to frozen 
sections and I'm having trouble with the testes cracking when I freeze 
them.  I've tried both freezing in isopentane cooled on liquid nitrogen 
and an acetone/dry ice slurry.  Either way the testes crack, usually one 
big crack end to end.  Doesn't seem to be as much of a problem with our 
mutant testes, which are about 1/3 the size of wild-type.  I wish I 
could just trim the wild-type down to a smaller size, but obviously 
that's not an option!

Any recommendations?

Thanks so much!


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