I've started attempting to section unfixed frozen mouse testes in order
to placate a specific antibody we have to use. I am new to frozen
sections and I'm having trouble with the testes cracking when I freeze
them. I've tried both freezing in isopentane cooled on liquid nitrogen
and an acetone/dry ice slurry. Either way the testes crack, usually one
big crack end to end. Doesn't seem to be as much of a problem with our
mutant testes, which are about 1/3 the size of wild-type. I wish I
could just trim the wild-type down to a smaller size, but obviously
that's not an option!
Thanks so much!
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