Re: [Histonet] mounting fluorescent tissue
|From:||"Markus F. Meyenhofer" |
a little light won't do any harm!
Just think of the high intensity of UV light you will bombard your samples
under the microscope !!!!
Markus F. Meyenhofer
----- Original Message -----
From: "Caroline Bass"
Sent: Thursday, September 21, 2006 10:19 AM
Subject: [Histonet] mounting fluorescent tissue
> Hi, I have what might be a silly question. I am dealing with a lot of
> native fluorescence, particularly in the brain, 40 um floating sections.
> At some point I want to mount the sections to observe the native
> fluorescence. I have been doing this in a dimly lit room, but it can be
> difficult at times. Do you think there would be a loss of signal if I
> mount the sections under normal light conditions? Also, if I choose to
> do immunofluorescence should I be careful, I heard that FITC staining
> should be done in the dark and I assume the mounting should as well.
> Any advice is appreciated. Thanks, Caroline
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