Reduce the dehydration steps to a minimum, starting with 95% EthOL and make them as fast as possible. Clearing will pose no problems for decolorization, but dehydration will.
  René J.

Gudrun Lang  wrote:
  Hi histonetters,
I am looking for a successful procedure for May-Grunwald-Giemsa stain on
cytological preps. My colleages in the cyto-lab want to establish it on an
automated stainer. They are interested in the right dehydration steps
afterwards.

Thanks in advance
Gudrun Lang


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