Re: [Histonet] VCAM-1, ICAM-1, P-selectin antibody

From:Gayle Callis

We buy our antibodies from BD Pharmingen, and do only frozen sections on 
fresh snap frozen tissues.  You can do either enzyme immunohistochemistry 
or delightful double immunfluorescence staining if you want to see 
colocalization of the antigens.   These antibodies may NOT work on formalin 
fixed paraffin seftions.

Go to BD Pharmingens website for pricing,  quantities, and also product 
data sheets for particulars on these antibodies.

At 02:03 AM 9/28/2006, you wrote:
>I need some recomendation to choose antibody for VCAM-1, ICAM-1 and 
>P-selectin detection on mouse vascular tissue either processed as frozen 
>or paraffin embedded section.  I found so many available products and I 
>have difficulties in choosing. If anyody have experience, please give me info.
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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