Re: [Histonet] Re: effects of elevation on immunohistochemistry

From:Rene J Buesa

Lisa:
  This is a Physics issue! At higher elevation atmosferic pressure is lower and the water boils at less than 100║C so any HIER done in an "open" container, like heating in the MW oven or using a steamer or a water bath, will be affected because 100║C cannot be reached.
  HIER should be done in a pressurized environment (like a pressure cooker). There was a paper published in the Jorunal of Histotechnology not too long ago (I do not have the exact reference) dealing with Her-2 Neu tests at high elevation.
  Any step requiring a certain temperature in an open container, will be affected. Other steps temperature independednt will not be affected.
  Hope this will help you!
  RenÚ J.

Lisa Manning  wrote:
  Good morning, 
Does anyone have any experience or knowledge about the effects of
elevation on immunohistochemistry?
Thanks kindly, Lisa Manning

Lisa Manning R.T, B.Sc.
Histopathology Biologist
Canadian Food Inspection Agency
National Centre for Foreign Animal Disease
1015 Arlington Avenue, Suite T2300
Winnipeg, Manitoba R3E 3M4
PH. (204) 789-2087
FAX (204)789-2038
email: manningl@inspection.gc.ca
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