Re: [Histonet] Honey as a fixative?
I would be very surprised if there is anything in honey that acts as
a fixative. Remember that honey is evolved to be food for growing bee
larvae, not exactly something compatible with fixation.
I do find it easy to believe that honey acts as a preservative,
simply because its high sugar content makes honey a strong
dehydrating agent. This will perserve tissue and prevent bacterial
and fungal growth. It would be interesting to do a similar study on
salt-preserved tissues. I suspect the results would be similar,
although the morphology would be uglier in the salt-preseved tissue
because salt's strong osmotic effects.
> I saw an article in the Journal of Histotechnology on honey as a
>fixative and was impressed by the innovative spirit of the authors.
> It would be helpful to the whole histo community if more studies
>are done on honey as a fixative.
> The main question should be if the results are reproducible by
>other investigators using honey from different areas. It would be
>also interesting to know the component of honey that is responsible
>for the fixation.
> James Mubiru
Microscopy Facility Supervisor
Central Michigan University
024C Brooks Hall
Mt. Pleasant, MI 48859
voice: (989) 774-3576
dept. fax: (989) 774-3462
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