Re: [Histonet] Antigen retrieval in the microwave

From:Phil McArdle

If you'll forgive a vendor for butting in...

Regarding the microwave pressure cooker: Let's not confuse "microwave 
transparency" with what we typically associate with the concept of 
transparency: /optical/ transparency. They're not the same. Microwave 
transparency refers to an object's ability to allow passage of microwave 
energy without converting a significant portion to heat. While some 
optically transparent objects happen also to be microwave transparent, 
that's not always true; leaded crystal in a microwave would be a 
disaster. Conversely, opaque white Teflon isn't something we'd think of 
as "transparent," yet it IS "microwave transparent."

EBS has just produced a "Microwave Companion for Histology," a 20-page 
booklet that seeks to put to rest just this kind of confusion, and it's 
free for the asking. It's purely informational, not looking to sell 
anything, with a view towards "advancing the art" rather than "shameless 
commerce." Please contact me directly and I'll be happy to send you one, 
or a few.

Best regards,

Phil McArdle

Microwave Product Manager
Energy Beam Sciences, Inc.
Tel:  800.992.9037 x 341
Fax: 860.653.0422

Terri Braud wrote:
> The trick will be to only use transparent containers, another CAPmania microwave pressure cooker just got 86'd.  I would not consider citrate buffer to be Hazardous.....good grief, I have boiled salt water in mine at home, and have lived to tell the tale.  Terri
> Terri L. Braud, HT(ASCP)
> Anatomic Pathology Supv.
> Laboratory
> Holy Redeemer Hospital
> (215) 938-3689
> -----Original Message-----
> From:
> []On Behalf Of Angela
> Bitting
> Sent: Tuesday, September 26, 2006 2:24 PM
> To:
> Subject: [Histonet] Antigen retrieval in the microwave
> I want to make sure I'm, totally CAP compliant, soooooo here's my
> question.
> Has anyone gotten cited for heating their citrate buffer or EDTA (for
> antigen retrieval)  in an unvented microwave? 
> I'm getting the impression that CAP wants all microwaves vented even
> though they throw out that blurb about "unnecessary if only
> non-hazardous solutions are being heated, i.e. water, some biological
> stains, paraffin sections".
> Angela Bitting, HT(ASCP)
> Technical Specialist, Histology
> Geisinger Medical Center 
> 100 N Academy Ave. MC 23-00
> Danville, PA 17822
> phone  570-214-9634
> fax  570-271-5916
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