RE: [Histonet] honey as a formalin substitute

From:"Truscott, Tom"

I would also be curious to know if honey collected from plants high in estrogens would affect any type of estrogen staining after processing tissue fixed in honey. Tom Truscott

-----Original Message-----
From: [] On Behalf Of Rene J Buesa
Sent: Thursday, September 21, 2006 8:30 AM
To: Olszewski, Dawn;
Subject: Re: [Histonet] honey as a formalin substitute

  The article was published in the Journal of Histotechnology, September 2006 issue (vol.29, No.3, pages 173-9).
  I read the article and was not really impressed by it because:
  1-the honey used came from a source that would not be readily available to everybody, which means that tests with other types/sources of honey will be necessary;
  2-there are no comparative methods, no statistical analysis of the results or documented comparisons with standard procedures, and
  3- if to substantiate the results we have to judge by the photomicrographs, they are of a very poor quality and what reflect is poor processing. That poor processing could be due to the processing protocol itself or to the fixation the authors claim to have perfomed with honey.
  If that is the case, the procedure does not seem to be very promising. I would have require the authors to rewrite some aspects of the paper before publication.
  Just my opinion based in what I read.
  René J.

"Olszewski, Dawn"  wrote:
We have a student in our lab who has been asked to write a summary of the article "The effectiveness of honey as a substitute for formalin in the histological fixation of tissue". Has anyone read this article ( or know where this article can be found) or know anything about this subject matter? If so, any and all info would be appreciated. Thanks in advance. 

Dawn Olszewski
Valdosta, Ga

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