RE: [Histonet] Yeh glad to be back & ??

From:"Patsy Ruegg"

I agree with Liz on this, it is hard enough with Tunnel to distinguish
between necrosis and apoptosis without adding decal.  I much prefer caspase,
you can count on the positive cells being just those under going apoptosis.

-----Original Message-----
[] On Behalf Of Liz Chlipala
Sent: Thursday, September 21, 2006 9:45 AM
To: 'Amy Porter'; 'Histonet'
Subject: RE: [Histonet] Yeh glad to be back & ??


I did this back a few years ago and found that the process of formic acid
decalcification caused all the nuclei to be positive.  From what I remember
the use of formic acid is not compatible with the tunel technique.  Why
don't you try using cleaved caspase 3 immunohistochemistry instead.


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 735-5001
fax (303) 735-3540
Ship to Address:
Premier Laboratory, LLC
University of Colorado at Boulder
MCDB, Room A3B40
Boulder, CO 80309

-----Original Message-----
[] On Behalf Of Amy Porter
Sent: Thursday, September 21, 2006 7:09 AM
To: Histonet
Subject: [Histonet] Yeh glad to be back & ??

Is anyone out there doing Tunel on FFPE acid decalcifed sections.  I am
trying to stain for apoptotic cells using a Tunel kit on decalcified mouse
femurs.  Not having much success, if anyone has any tips or do's / don'ts I
would really appreciate the information.  Thanks in advance, 

Amy S. Porter, HT(ASCP) QIHC - Supervisor
Michigan State University
Investigative HistoPathology Laboratory
Department of Physiology / Division of Human Pathology
2100 Biomedical Physical Sciences Bldg. Room #2133
East Lansing, MI  48824-3320
Phone:  (517) 355-6475 ext 1480 / Fax:  (517) 432-1368

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