RE: [Histonet] WBC counts

From:Gayle Callis

We purchase hemocytometers from Fisher  (Healthcare catalog) or VWR.  We 
still use them for lung lavage/washings from mice.  AND we do cell count 
aka differentials on Diff Quik (if you can get it without difficulty) 
stained murine lung lavage/cytospins.  If you have to do a differential on 
animal blood smears, buffy coats, Wright Giemsa stain is superb for this 
purpose, Harleco brand.

For a WBC count, contact a clinical laboratory to run the WBC counts on 
their automated counters, much more accurate and worth the cost.  It was 
painful to work with the RBC and WBC pipettes - HOORAY for modern 
technology and instrumentation.

At 01:38 PM 9/26/2006, you wrote:
>Good luck finding a hemacytometer and/or any calibrated pipettes.  Ours
>ended up in the display of "antique instruments and equipment".  That's
>the best place for them (speaking as an old dinosaur who actually
>learned how to use them).

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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