Missy,

Is what your supervisor wants a WBC Differential? If so you count 100
cells identifying each different type of cell and report the percentage
of each cell type. A white blood cell count would have to be performed
on a whole blood specimen - not a peripheral smear - there is no way to
measure volume otherwise as cell counts are reported as number of cells
per volume measured.

Hope this helps.

Dave Haagsma MT(ASCP)


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Missy
Sent: Tuesday, September 26, 2006 10:25 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] WBC counts

Hi all...
We have never done a manual WBC count before and my advisor has asked me
to
get a count of WBCs (primarily nuetrophils and lymphocytes) in wright's
stained peripheral blood smears.  What is the general protocol for a
manual
count? Do you count WBC's inb relation to RBCs or just WBCs, the whole
slide, or just representative sections?

Melissa

P.S - this is for rat sample, not human
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