We have done both of these things and have not noticed a difference. When 
the tissues were finally embedded and cut they were fine.
Andi




At 09:24 AM 9/27/2006 -0700, you wrote:
>I prefer to place the cassettes in a low carboard box of adequate size, 
>filled with melted paraffin, and to make like a gigantic "common" paraffin=20
>block for all, that will be melted as later on to prepare the final 
>individual tissue blocks.
>   René J.
>
>Tony Henwood  wrote:
>   Since the tissue is already processed to wax, you can safely remove the
>cassettes from the wax and let them harden to room temp. Then to embed,
>place them in molten wax for 30 min or so to remelt the tissue ready for
>embedding. The tissue is already infiltrated with wax so no harm can
>come to them.
>
>Regards
>
>Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
>Laboratory Manager & Senior Scientist
>The Children's Hospital at Westmead,
>Locked Bag 4001, Westmead, 2145, AUSTRALIA.
>Tel: 612 9845 3306
>Fax: 612 9845 3318
>
>
>
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
>Heckford, Karen - SMMC-SF
>Sent: Tuesday, 26 September 2006 10:21 PM
>To: Histonet (E-mail)
>Subject: [Histonet] Tissue in Paraffin
>
>
>Good Morning my fellow Histonetters, I was wondering how long you would
>leave tissue unembedded after pulling them out of paraffin. My
>processor is broke down. I would greatly appreciate the feedback.
>Cheers,
>Karen Heckford HT (ASCP) CE
>Lead Histology Technician
>Histology/Pathololgy Department
>St. Mary's Medical Center
>450 Stanyan St.
>San Francisco, Ca. 94117
>415-668-1000 ext. 6167
>Fax: 415-750-8123
>email: kheckfor@chw.edu
>
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: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
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