RE: [Histonet] Tissue in Paraffin

From:Rene J Buesa

I prefer to place the cassettes in a low carboard box of adequate size, filled with melted paraffin, and to make like a gigantic "common" paraffin block for all, that will be melted as later on to prepare the final individual tissue blocks.
  René J.

Tony Henwood  wrote:
  Since the tissue is already processed to wax, you can safely remove the
cassettes from the wax and let them harden to room temp. Then to embed,
place them in molten wax for 30 min or so to remelt the tissue ready for
embedding. The tissue is already infiltrated with wax so no harm can
come to them.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of
Heckford, Karen - SMMC-SF
Sent: Tuesday, 26 September 2006 10:21 PM
To: Histonet (E-mail)
Subject: [Histonet] Tissue in Paraffin

Good Morning my fellow Histonetters, I was wondering how long you would
leave tissue unembedded after pulling them out of paraffin. My
processor is broke down. I would greatly appreciate the feedback.
Karen Heckford HT (ASCP) CE
Lead Histology Technician
Histology/Pathololgy Department
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
Fax: 415-750-8123

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