RE: [Histonet] Fatty unfixed tissues?

From:"Tony Henwood"

The best process I've found is to gently squeeze out the excess wax,
place back into the cassette and return to 10% formalin. Reprocessing
seems to fix the problem.
Reference below:

While the tissue is still hot (ie the wax is still molten) blot the
tissue dry, place it back in its cassette and place the cassette in 10%
formalin for reprocessing. I have often had recourse to use this method
and have found it to dramatically improve the results in at least 90% of
cases. The excellent results are probably due to the protective nature
of the wax present in the adequately processed portions of the block.
This insulates the tissue from the harmful effects of ethanol on the
adequately processed portions of the tissue preventing the tissue from
becoming hard and brittle (Johnson (2003) Histologic 36(1):21-22).

Regards

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318




-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of sheila
adey
Sent: Tuesday, 26 September 2006 10:12 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Fatty unfixed tissues?


Hi Everyone,

Just wondering if anyone has any neat ideas about what to do with fatty 
tissue that comes out of the processor not processed. We have done the 
"squeeze" technique and then re soak in paraffin. That helps but any
other 
ideas? It's one of those pathologists who has to put everything in the
day 
it arrives and is not a thin slicer.

Thanks in advance

Sheila

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