[Histonet] mounting fluorescent tissue
Hi, I have what might be a silly question. I am dealing with a lot
of native fluorescence, particularly in the brain, 40 um floating
sections. At some point I want to mount the sections to observe the
native fluorescence. I have been doing this in a dimly lit room, but
it can be difficult at times. Do you think there would be a loss of
signal if I mount the sections under normal light conditions? Also,
if I choose to do immunofluorescence should I be careful, I heard
that FITC staining should be done in the dark and I assume the
mounting should as well.
Any advice is appreciated. Thanks, Caroline
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