[Histonet] Re: Histonet Plasmacytosis
We use CD138 pre-dilute from Cell Marque on the Ventana Benchmark XT. They
have several different sizes of pre-dilute and concentrate. Our
pathologists love it. We use hidradenitis suppurativa as a positive
control (it has a lot more plasma cells than tonsil and is easier to get
Mild cell conditioning (Tris/EDTA buffer, pH 8) for 30 minutes.
AB incubation 32 min @ 37 degrees.
Hope this helps.
Date: Tue, 19 Sep 2006 13:07:38 -0400
From: "Weems, Joyce"
Subject: [Histonet] Immunoperoxidase for plasmacytosis
To: "Histonet \(E-mail\)"
Content-Type: text/plain; charset="utf-8"
We have a pathologist who is insisting on a stain for plasma cells. Thus
the previous question about MGP kits.
Second question, do any of you do CD138 for plasma cells. If so, would you
please share your source for the antibody and your procedure?
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
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