Re: [Histonet] Control tissue for skin DIF

From:"Joe Nocito"

if you find a source, please let me know.  We save cases that stain 
positive, but we don't have a full spectrum of known controls. Our dermpath 
is content with that (no, really, this is a huge, huge point, this lady is 
not content with anything else). I've contacted several sources. They do not 
or will not commit to storing frozen section slides and still guarantee 
their antigenicity, which I can't blame them because our own controls fade 
after a while.
    I know this wasn't that helpful, but hell, it's Friday, so let's enjoy 
the weekend.

Histology Manager
Pathology Reference Lab
San Antonio, TX
----- Original Message ----- 
From: "Luke A Perkocha" 
Sent: Friday, September 08, 2006 8:42 AM
Subject: [Histonet] Control tissue for skin DIF

>   Hello,
>   I  am trying to develop a protocol for doing direct immunofluorescence
>   testing  on  skin  for  bullous  disease.  I  have  a couple of sample
>   protocols  but  none  say  anything about positive control tissue. I'm
>   uncomfortable running the test on clinical specimens and reporting out
>   a  negative,  without  good  positive  controls. Does anyone know of a
>   commercial  source  for  positive  control tissue, or does anyone have
>   some  they  would  be  willing  to  share  for  development? Help much
>   appreciated.
>   Thanks,
>   Luke
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