Karen:
  Yours is a simple case: if your smears were NOT fixed with formalin, you do NOT need to do HIER (and that is what I think you refer to when writing about the buffer).
  To do IHC in alcohol fixed smears, you just bring them to water, then to the PBS and start the IHC procedure without the HIER step (solely designed to eliminate the crosslinkage due to the NBF fixation).
  Hope this will help you!
  René J.

"Heckford, Karen - SMMC-SF"  wrote:
  Hello Everyone, I have never had to do IHC on cell smears only FFPE. I am
not sure how to bring them down to buffer before placing them on the
Autostainer. Is there something special I need to do? The smears are in 95%
alcohol. Do I just place them straight into the buffer? Do I need to rinse
them? Augh!!!! HELP!!! 
Cheers,
Karen Heckford HT (ASCP) CE
Lead Histology Technician
Histology/Pathololgy Department
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
Fax: 415-750-8123
email: kheckfor@chw.edu
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