RE: [Histonet] sections cutting thick and thin

From:"Zajic Kari"

Hi Gerard...yes, I have experienced that problem as well with my Leica Microtome. It seems that the "plates" that hold the back of the knife holder kind-of warp...I am sorry, I don't know the technical terms (my service guy always knew what I was referring to). They seem to warp after alot of cutting (I used to cut alot of uterus,bone,etc) and I would have them replaced about every 18 months or so or as soon as I experienced the thick/thin sections. I wish I could explain the part a little better for you!! If you take off the back plate of the knife holder, you'll see a small "square" with 2 thin metal clamps. Those are the culprits! Good luck, I know how frustrating they are!!

Kari :) 

Kari Marie Zajic HTL,MLT
Histology Supervisor
Palms West Hospital
Pathology Department
13001 State Road Eighty
Loxahatchee, Florida 33470
phone:      (561)798-6036
telefax:     (561)753-4298
voicemail: (561)753-4299
pager:       (561)610-4949

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-----Original Message-----
[]On Behalf Of Gerard
Sent: Thursday, September 07, 2006 10:23 PM
Subject: [Histonet] sections cutting thick and thin

Dear Histonetters, 
Recently our Leica 2135 microtome has been giving us problems. It had only just been serviced and started to cut thick and thin. We've isolated it to the knife holder, as we exchanged the knife holder from a more recent Leica microtome and it was fine. Has anyone with this model had the same problem?
Gerard Spoelstra
Medical Scientist
Murdoch University
Western Australia  
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