You would most likely have no problem transferring smears from 95% alcohol
directly into buffer, but when I do such preps I err on the side of caution
and place them in 70% alcohol for a few minutes before going into buffer.

> ----------
> From: 	histonet-bounces@lists.utsouthwestern.edu on behalf of
> Heckford, Karen - SMMC-SF
> Sent: 	Thursday, September 7, 2006 10:51 AM
> To: 	Histonet (E-mail)
> Subject: 	[Histonet] Cell Smears
> 
> <>
> Hello Everyone,  I have never had to do IHC on cell smears only FFPE.  I
> am
> not sure how to bring them down to buffer before placing them on the
> Autostainer. Is there something special I need to do?  The smears are in
> 95%
> alcohol.  Do I just place them straight into the buffer?  Do I need to
> rinse
> them?  Augh!!!! HELP!!!  
> Cheers,
> Karen Heckford HT (ASCP) CE
> Lead Histology Technician
> Histology/Pathololgy Department
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> Fax: 415-750-8123
> email: kheckfor@chw.edu
> 
> 

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