RE: [Histonet] Anti mouse CD31
From: | "Bugelski, Peter [CNTUS]" |
I've had good luck with murine anti-CD31 in paraffin sections of human
xenografts in tissue fixed with a modification of Beckstead's zinc.
Tris 12.1 g
Calcium chloride 0.5 g
Zinc acetate 10 g
formalin 13.5 mL
Distilled H2O to 900 mL
adjust pH until clear
Bring to 1000 mL
We published using this this fixative in Tang Y et al. CAncer Res 65:3193,
2005
Peter J. Bugelski, Ph.D., FRCPath
Senior Research Fellow and
Head of Experimental Pathology
Centocor R-4-2
200 Great Valley Parkway
Malvern, PA 19355
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
<< Previous Message | Next Message >>