[Histonet] re: Co-localization studies...
If you are using direct immunofluorescence, two primaries from the same
species should not matter as long as they are fluorescently labeled
differently. However, if you are using indirect immunofluroescence, the
secondary antibody would recognize both primaries and you could not tell
which stain was which.
Melville B. Vaughan, Ph. D.
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
I am a little bit ashamed, but I have a very basic question to put you:
are doing co-localization studies (IF) both primary antibodies should not
raised in the same species. Why? What would be the result? I would like to
a little bit more about this subject, where should I look for?
R. do Campo Alegre, 823
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