Fatima,
If you are using direct immunofluorescence, two primaries from the same 
species should not matter as long as they are fluorescently labeled 
differently. However, if you are using indirect immunofluroescence, the 
secondary antibody would recognize both primaries and you could not tell 
which stain was which. 
Mel
Melville B. Vaughan, Ph. D.
Assistant Professor
Department of Biology
University of Central Oklahoma
100 N. University Drive
Edmond, OK 73034
http://www.biology.ucok.edu/PersonalPages/mvaughan/Default.htm

Hello Histonetters,

I am a little bit ashamed, but I have a very basic question to put you: 
when we
are doing co-localization studies (IF) both primary antibodies should not 
be
raised in the same species. Why? What would be the result? I would like to 
read
a little bit more about this subject, where should I look for?

Thank you,

Fátima


-- 
Fátima Ferreirinha
IBMC
R. do Campo Alegre, 823
4150-180, Porto
Portugal
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet