[Histonet] Histology Openings

From:"Kopczynski, Charlotte"

 BayCare Health System, serving the Tampa Bay Area, has openings for:

Histotechnicians/Histotechnologists....

Current Florida license and ASCP certification required. Performs routine histology procedures in a fully automated laboratory. Other duties include responsibility for instrumentation operation and maintenance. Record documentation, quality control monitoring, computer applications and lab safety requirements are also needed. Strong interpersonal and excellent customer service skills. Good communication skills.

Contact:  BayCare Regional Services
E-mail:  regional.teamresources@baycare.org
16255 BayVista Drive
Clearwater, FL 33760
Phone (866) 221-3222
Apply On Line - http://baycare.org

Company Profile

The Tampa Bay area's leading not-for-profit hospitals, BayCare Health System is a joint operating agreement between three Community Health Alliances (CHAs). Each CHA
uniquely focuses on the distinctive needs of their communities and taps into the regional
system for improved quality, access and efficiencies. The CHAs are:
*	St. Anthony's Health Care, which includes St. Anthony's Hospital in St. Petersburg.
*	Morton Plant Mease Health Care, which includes Morton Plant Hospital in
Clearwater and Morton Plant North Bay Hospital in Pasco County. Mease
Countryside and Mease Dunedin hospitals. 	
*	St. Joseph's-Baptist Health Care, which includes St. Joseph's, St. Joseph's
Children's, and St. Joseph's Women's hospitals in Tampa and South Florida Baptist Hospitals in Plant City.



Thanks,
Charlotte Kopczynski,HTL(ASCP)
Regional Pathology Manager
BayCare Laboratories
Phone: 727-461-8246
Fax:   727-462-7596


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of histonet-request@lists.utsouthwestern.edu
Sent: Tuesday, September 05, 2006 1:10 PM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 34, Issue 5

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Today's Topics:

   1. HSRL Job Opportunities (HSRL)
   2. RE: Co-localization studies... (Johnson, Teri)
   3. Sakura Coverslipper Timing (Chris Pomajzl)
   4. lighted floatation Bath (Steven Coakley)
   5. Re: lighted floatation Bath (Kathleen Roberts)
   6. Phoenix (Andrea Grantham)
   7. Re: lighted floatation Bath (Markus F. Meyenhofer)
   8. RE: lighted floatation Bath (Bartlett, Jeanine (CDC/CCID/NCID))
   9. RE: lighted floatation Bath (David Haagsma)
  10. Anyone looking for an Ultra Low Freezer (Amber Tyler ANTYLER)
  11. FW: Re:  Collagen stain for paraffin sections
      (Sherwood, Margaret )
  12. If You Can't Go to NSH in AZ We Have an Alternative in
      October (Pamela Marcum)
  13. Peloris tissue processor (lhartman18@adelphia.net)
  14. QUALIFICATIONS FOR SUPERVISOR (Vickroy, Jim)
  15. RE: Peloris tissue processor (Douglas D Deltour)


----------------------------------------------------------------------

Message: 1
Date: Mon, 4 Sep 2006 15:15:25 -0400
From: "HSRL" 
Subject: [Histonet] HSRL Job Opportunities
To: 
Message-ID: <000701c6d056$7d12b260$0700a8c0@HSRLMAIN>
Content-Type: text/plain;	charset="iso-8859-1"

Dear Netters,

 

Histo-Scientific Research Laboratories (HSRL, Inc.) is looking to add two full time histotechs and one part time histotech to our team.  HSRL is a privately-owned histopathology laboratory located in the Shenandoah Valley of Virginia.  We are about 1 ½ hours SW of Washington DC tucked away in the beautiful Blue Ridge Mountains.  The ideal candidate would have skills in basic paraffin histology as well as plastics (GMA and/or
MMA) histology.   We offer a full benefits package along with
competitive wages, paid vacation days, and a FRIENDLY and RELAXED ATMOSPHERE!  

 

If you are interested in learning more about the opportunities at HSRL, please fax or e-mail resume to:

 

Tom Galati

Laboratory Director

HSRL, Inc.- A GLP Compliant Contract Laboratory

5930 Main Street

Mount Jackson, Virginia  22842

540.477.4440

FAX:  540.477.4448

tomgalati@hsrl.org

www.hsrl.org

 

 

Thank you.



------------------------------

Message: 2
Date: Mon, 4 Sep 2006 18:14:44 -0500
From: "Johnson, Teri" 
Subject: [Histonet] RE: Co-localization studies...
To: 
Message-ID:
	
	
Content-Type: text/plain; charset="iso-8859-1"

>>
I am a little bit ashamed, but I have a very basic question to put you: when we are doing co-localization studies (IF) both primary antibodies should not be raised in the same species. Why? What would be the result? I would like to read a little bit more about this subject, where should I look for?

Thank you,

Fátima<<

Fatima - you can do this easily if you have abundant protein and directly labeled antibodies. However, if you are using unlabeled primary antibodies, you need a secondary antibody to complex with the first which adds either an enzyme, biotin, or a fluorescent label. The secondary antibody is usually targeted against the animal species the first antibody was made in. Therefore, if both your primary antibodies are rabbit, and you use a green fluorescent labeled anti-rabbit and a red fluorescent labeled anti-rabbit, both labels will bind with the rabbit species. Everywhere both antibodies bound with the target protein will label with both red and green.

Even though that will show colocalization, it does not give you the option of seeing each color channel separately. What if your antigens do not colocalize in every cell? It will look as though it does by doing your immunostaining this way. If primary antibody #1 is located in cell type A and cell type C, and primary antibody #2 is located in cell type B and cell type C, they should only co-localize in C. But by doing it with your method, you will also show colocalization in cell types A, B, and C.

Does this make sense?

If you use two primary antibodies raised in different species, say goat for Antibody #1 and rabbit for Antibody #2, you can use an anti-goat green fluorescent labeled secondary antibody, and an anti-rabbit red fluorescent labeled secondary antibody. The anti-goat should localize your Antibody #1, and the anti-rabbit should localize your Antibody #2. Therefore, given the above example, Antibody #1 will show positivity in cell type A and C, Antibody #2 will show positivity in cell type B and C, and they will only co-local
ize in cell type C.

For more information on multiple immunostaining methods, I urge you to purchase and read (cover to cover) Chris van der Loos' book, ISBN 038791594X.

Best wishes,

Teri Johnson
Stowers Institute for Medical Research
Kansas City, MO




------------------------------

Message: 3
Date: Tue, 5 Sep 2006 06:34:00 -0500
From: "Chris Pomajzl" 
Subject: [Histonet] Sakura Coverslipper Timing
To: "HISTONET" 
Message-ID: <001a01c6d0df$2f491180$26fca8c0@CSP>
Content-Type: text/plain;	charset="iso-8859-1"

Histonetters:

We have 2 Sakura coverslippers that are in desperate need of some TLC, maintenance and repair. They are 7+ years old, and have been used and abused for all of those years. They are shared equipment between Histo and Cytology. Therefore, there has been 7 years of PAP juice buildup. Our in-house BioMed tech retired a couple of years ago, and ever since, we have used a local BioMed company that does not have much experience with these machines. They would come out and temporarily fix any problems, but it would only correct the problem for a week or two.

As a result I have to do much of the maintenance and repair myself. I am somewhat mechanically inclined, but I have my limits. I finally disassembled one of the machines last week so that I could remove all of the disgusting build-up that was covering all of the parts. I was able to put it back together, and it appears to be in good working order, except for one problem. I cannot get the timing of the solenoid in sync with the lever arm that allows the receiving basket to drop one notch of the basket at a time. The service manual explains how to fix the timing, but I don't know that I understand these directions clearly. It just doesn't make much sense, and I simply am not sure I am doing it correctly.

Does anyone have any experience who could offer some tips and advice? It would be much appreciated. We are in the process of replacing one of these machines, but that will take some time, I'm sure. And that still leaves me with one old machine.


Chris Pomajzl, BS, HTL (ASCP)

Histology Supervisor
Clinical Pathology Laboratories, Inc.
9200 Wall Street
Austin, Texas 78754
512.873.1660 (o)
512.873.5004 (f)
cpomajzl@cpllabs.com

CONFIDENTIALITY NOTICE:  This communication is intended only for the use of the individual or entity to which it is addressed and may contain information that is privileged, confidential, and exempt from disclosure under applicable law.  If you are not the intended recipient, you are notified that any use, dissemination, distribution, or copying of this communication is strictly prohibited. If you have received this message in error, please notify the sender immediately and destroy this communication.


------------------------------

Message: 4
Date: Tue, 5 Sep 2006 06:41:42 -0700 (PDT)
From: Steven Coakley 
Subject: [Histonet] lighted floatation Bath
To: Histonet@lists.utsouthwestern.edu
Message-ID: <20060905134142.5601.qmail@web38209.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

I'm looking for a working lighted floatation bath as a primary for our lab.  
 		
---------------------------------
How low will we go? Check out Yahoo! Messenger's low  PC-to-Phone call rates.

------------------------------

Message: 5
Date: Tue, 05 Sep 2006 09:52:37 -0400
From: Kathleen Roberts 
Subject: Re: [Histonet] lighted floatation Bath
To: Steven Coakley 
Cc: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID: <44FD8125.3020403@rci.rutgers.edu>
Content-Type: text/plain; charset=windows-1252; format=flowed

I like this one from Fisher Scientific: 
https://www1.fishersci.com/Coupon?cid=1328&gid=137998

Kathleen Roberts
Neurotoxicology Labs
Rutgers University

Steven Coakley wrote:

>I'm looking for a working lighted floatation bath as a primary for our lab.  
> 		
>---------------------------------
>How low will we go? Check out Yahoo! Messenger's low  PC-to-Phone call rates.
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>



------------------------------

Message: 6
Date: Tue, 05 Sep 2006 06:58:10 -0700
From: Andrea Grantham 
Subject: [Histonet] Phoenix
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<4.3.2.7.2.20060905065316.00c7f678@algranth.inbox.email.arizona.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

This week many of you will be traveling to Phoenix for the 32nd NSH Symposium/Convention and the Arizona Committee is anxiously awaiting for your arrival. We hope that you have a wonderful visit to Phoenix and Arizona. We have ordered cooler temperatures for you and maybe a bit of late summer monsoons. Please stop by the Phoenix PR table in the Convention Center to say hello.
Safe travels!

.....................................................................
: Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
: Sr. Research Specialist       University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
:...................................................................:
           http://www.cba.arizona.edu/histology-lab.html




------------------------------

Message: 7
Date: Tue, 5 Sep 2006 10:03:51 -0400
From: "Markus F. Meyenhofer" 
Subject: Re: [Histonet] lighted floatation Bath
To: "Steven Coakley" ,
	
Message-ID: <082d01c6d0f4$1e1b2190$26893cd1@DJ4VDH31>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

Several available, also other histo and EM equipment.
Please contact me off list.
Regards,
Markus F. Meyenhofer
Microscopy Labs
Box 338
61 West Street
Red Bank, NJ 07701
732 747 6228
fax 732 758 9142
----- Original Message -----
From: "Steven Coakley" 
To: 
Sent: Tuesday, September 05, 2006 9:41 AM
Subject: [Histonet] lighted floatation Bath


> I'm looking for a working lighted floatation bath as a primary for our 
> lab.
>
> ---------------------------------
> How low will we go? Check out Yahoo! Messenger's low  PC-to-Phone call 
> rates.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 




------------------------------

Message: 8
Date: Tue, 5 Sep 2006 09:56:56 -0400
From: "Bartlett, Jeanine \(CDC/CCID/NCID\)" 
Subject: RE: [Histonet] lighted floatation Bath
To: "Steven Coakley" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

I LOVE the one Surgipath sells.  It doesn't have the glass insert so it
heats very rapidly and has a black background. You want the square one,
not the round one.  It's product number  04535S.


Jeanine Bartlett, BS, HT(ASCP)
Centers for Disease Control and Prevention
1600 Clifton Road, MS/G-32
18/SB-114
Atlanta, GA  30333
(404) 639-3590 
jeanine.bartlett@cdc.hhs.gov

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Steven
Coakley
Sent: Tuesday, September 05, 2006 9:42 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] lighted floatation Bath

I'm looking for a working lighted floatation bath as a primary for our
lab.  
 		
---------------------------------
How low will we go? Check out Yahoo! Messenger's low  PC-to-Phone call
rates.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





------------------------------

Message: 9
Date: Tue, 5 Sep 2006 10:06:47 -0400
From: "David Haagsma" 
Subject: RE: [Histonet] lighted floatation Bath
To: "Kathleen Roberts" ,	"Steven Coakley"
	
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<19E3602A16438E48B51A4250CA04B5F680AE9B@exchange.marketlab.com>
Content-Type: text/plain;	charset="us-ascii"


Barnstead has a much newer model with LED temperature readout, separate
light and power switches and a temp probe for less money.

http://www.barnsteadthermolyne.com/search_modelnbr.cfm

Product Group:Histology
Product Category:TISSUE FLOAT BATH
Model Number: 26106Q

You have to plug the model number into the search field to see the
product.

Dave Haagsma 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kathleen
Roberts
Sent: Tuesday, September 05, 2006 9:53 AM
To: Steven Coakley
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: Re: [Histonet] lighted floatation Bath

I like this one from Fisher Scientific: 
https://www1.fishersci.com/Coupon?cid=1328&gid=137998

Kathleen Roberts
Neurotoxicology Labs
Rutgers University

Steven Coakley wrote:

>I'm looking for a working lighted floatation bath as a primary for our
lab.  
> 		
>---------------------------------
>How low will we go? Check out Yahoo! Messenger's low  PC-to-Phone call
rates.
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>  
>

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Tue, 5 Sep 2006 11:41:11 -0400
From: Amber Tyler ANTYLER 
Subject: [Histonet] Anyone looking for an Ultra Low Freezer
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
Content-Type: text/plain; charset="US-ASCII"

Just posting to tell everyone that New Brunswick Scientific has a discount 
offer on Ultralow freezers. I just received a quote today for half price.

Thank you, 
Amber N. Tyler, Ph.D.
Research Scientist
Dept of Psychology (PO Box 26170)
296 Eberhart Building, Walker Avenue
University of North Carolina - Greensboro
Greensboro, NC 27402





------------------------------

Message: 11
Date: Tue, 5 Sep 2006 11:54:14 -0400
From: "Sherwood, Margaret " 
Subject: [Histonet] FW: Re:  Collagen stain for paraffin sections
To: 
Message-ID:
	<1AF23D0AD12E7444A5DB083CA978B73407A30BC3@PHSXMB1.partners.org>
Content-Type: text/plain;	charset="iso-8859-1"

To all:
 
Does anyone know if the collagen stain, Masson Trichrome, would deliniate
denatured or necrotic collagen from normal collagen in paraffin sections?  One
of the researchers is looking for such a stain.  If not, is there another
collagen stain that would?  (We routinely use MTC).
 
Peggy

Peggy Sherwood 
Lab Associate, Photopathology 
Wellman Center for Photomedicine (W224) 
Massachusetts General Hospital 
55 Fruit Street 
Boston, MA 02114 
617-724-4839 (voice mail) 
617-726-6983 (lab) 
617-726-3192 (fax) 
msherwood@partners.org 

 


------------------------------

Message: 12
Date: Tue, 05 Sep 2006 12:15:25 -0400
From: Pamela Marcum 
Subject: [Histonet] If You Can't Go to NSH in AZ We Have an
	Alternative in	October
To: Histonet@lists.utsouthwestern.edu
Message-ID: <6.1.1.1.2.20060905115941.01a24ab8@mail.vet.upenn.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed



The Pennsylvania Histotechnology Society is holding an October seminar 
program in the Pittsburgh area.  If you could not go to the NSH Symposium 
in Phoenix and you will still need to complete CEUs this year join us for 
the meeting.  We will have 3 days of special seminars covering management 
issues, beginning and advanced IHC classes, forensics autopsy and 
entomology to name a few.

Go to our web site at www.pahisto.org for a full program and registration 
forms. The meeting will be in Cranberry, PA just outside Pittsburgh so we 
encourage members of NSH, Ohio, New York, West Virginia and all of Region 
II to join us in an educational/fun experience.  We will have vendor 
exhibits and a special workshop on automated special stainers they will 
participate in.

We have workshops and talks planned for all phases of Histology and many 
related fields.  We will have a talk on FNA procedures for 
cytology/histology.  We will have safety and autopsy workshops for PAs and 
others who will be required to have CEUs in these areas.

Plese contact me if you have any questions.

Best Regards,

Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr.  CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348

Phone - 610-925-6278
Fax     - 610-925-8120
E-mail - pmarcum@vet.upenn.edu 





------------------------------

Message: 13
Date: Tue, 5 Sep 2006 9:25:40 -0700
From: 
Subject: [Histonet] Peloris tissue processor
To: histonet@lists.utsouthwestern.edu
Message-ID: <6769591.1157473540991.JavaMail.root@web18>
Content-Type: text/plain; charset=utf-8

Would you please share your experiences with the Peloris tissue processor by visionbiosystems?  Any comments would be appreciated.
Thanks,
 
Linda Hartman
Histology Section Head
Mount Nittany Medical Center
State College, PA 16803
814-234-6117 X6650
email lhartman@mountnittany.org



------------------------------

Message: 14
Date: Tue, 5 Sep 2006 11:48:40 -0500
From: "Vickroy, Jim" 
Subject: [Histonet] QUALIFICATIONS FOR SUPERVISOR
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

 

Are there folks supervising histology laboratories that only have an HT
certification and not a degree?  And.............

What do the governing organizations say about this?     I know I can get
the information but wondered if anyone had a quick answer.

 

Jim Vickroy

Technical Supervisor - Surgical and Autopsy Pathology

Memorial Medical Center

 



This message (including any attachments) contains confidential information intended for a 
specific individual and purpose, and is protected by law. If you are not the intended recipient, 
you should delete this message. Any disclosure, copying, or distribution of this message, or the 
taking of any action based on it, is strictly prohibited.


------------------------------

Message: 15
Date: Tue, 5 Sep 2006 12:56:11 -0400
From: "Douglas D Deltour" 
Subject: RE: [Histonet] Peloris tissue processor
To: ,	
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"

Run away! Ask them for a list of users. I have one sitting in my storage
area waiting on them to pick it up. It has never worked right and the tech
support is non existent. The techs do not know how to fix it. It is the
second one that they have sent us. If anyone from Vision is reading this
please have it removed from our storage area. It has been here for four
months waiting on you to pick it up. I hope the acquisition of this company
doesn't tarnish the Ventana reputation. 


 
Douglas D. Deltour HT(ASCP)
Histology Supervisor
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
 
*****************************************************
PROFESSIONAL PATHOLOGY SERVICES, PC
NOTICE OF CONFIDENTIALITY
This message is intended only for the use of the individual or entity to
which it is addressed and may contain information that is privileged,
confidential and exempt from disclosure under applicable law. If the reader
of this message is not the intended recipient, you are hereby notified that
any dissemination, distribution, or copying of this communication is
strictly prohibited by law. If you have received this communication in
error, please notify me immediately.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of
lhartman18@adelphia.net
Sent: Tuesday, September 05, 2006 12:26 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Peloris tissue processor

Would you please share your experiences with the Peloris tissue processor by
visionbiosystems?  Any comments would be appreciated.
Thanks,
 
Linda Hartman
Histology Section Head
Mount Nittany Medical Center
State College, PA 16803
814-234-6117 X6650
email lhartman@mountnittany.org

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------------------------------

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