Okay...first, thanks to Pam Marcum of UPenn for directing me to a kit
available from Polysciences. Sorry if my original subject line was misleading.
What I want to do is figure out how long the decal solution continues to be
effective. My client is happy with their technique of determining when the
sample is sufficiently decalcified. Right now an adult rat femur is placed in a
50ml centrifuge tube with approximately 30 ml of EDTA. The tube is placed in
a rack on a stirrer, the solution is changed once a week. I was thinking if
they increased the volume of solution, or changed it more often, it would
take less time to completely decalcify the sample. Am I on the right the track?
Now I'm curious about the device that Dr. McCormick mentioned. Is anyone
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