Check on the expirartion date of the slides. After some months they don't work as they are supposed to. Slides should be drained/dried on edge (not flat). A last resort would be to coverslip by hand, looking at the edges (and even flattening them out with a hair brush before coverslipping).
Rene J. Buesa

Sam Vaughn  wrote:
I'm new to histonet and have a question about histo staining.
I have 6 um paraffin sections of mouse knee joints on uncoated superfrost
plus slides. I collect the sections from a distilled water bath and let them
drain at least 30min before placing them at 37 degrees overnight before
staining. After Safranin O/ Fast Green staining a very thin area around the
edge at the edge of the tissue lifts off the slide rendering it impossible
to visualize with the microscope. Of course this is the the articular
surface which we are interested in! I've used the same slides in the past
without any coating and it has worked fine. I'm in a new lab now and trying
to get things running. Any help you could give me would be really
appreciated!
Thanks,
Sam
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