Re: [Histonet] re JKiernan APES treatment of slides
1. I don't know how commercial positively charged
slides are made. Try asking the maker. (Good luck!)
It is possible that they use an alkoxysilane other
than APES to achieve a similar effect.
2. Using an acetone rinse to speed up drying should
not change the APES-treated slides. The aminopropyl
groups are covalently bound to the glass. They
cannot be washed off, and they do not react with acetone.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
Carl Hobbs wrote:
> Thank you very much for your reiteration of the procedure for charging
> > slides, using APES, Mr Kiernan. I would be grateful for your comments
> > regarding:
> > 1. Do you know if the "Superfrost plus" slides that I buy from VWR are
> > prepared using the same/similar process?
> > 2. I use a similar method to your posted one ; it differs only in that I
> > rinse the
> > slides in water after the APES/acetone treatment, then rinse in acetone
> > to aid a fast dry; do you think that this has any deleterious effect on
> > the charging of the slides?
> > Carl
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