Re: [Histonet] processing skin and intestines

From:Gayle Callis

Isn't the Citadel a carousel (sp?) style with vacuum on last station of 
paraffin?  I am not totally familiar with this processor.

With our VIP (vacuum/pressure throughout), 45 minutes per station of 
dehyrant, clearing and paraffin (60C) at most but we get superb exchange of 
solvents with VIP processing, and we never add heat to dehydration/clearing 
steps - dries out the tissue even more.

For rat, I would do 1 hour on VIP.   If you do not have vacuum and pressure 
throughout for each station,  then mouse tissue will survive 1 hour timing 
on a carousel style nicely.

Keep in mind animal are less fatty than human, and can easily be 
overdehydrated, dried out.

At 05:38 PM 9/27/2005, you wrote:
>Howdy folks!
>We've been asked by some scientists to process skin, cheek pouches and 
>intestines from mice for FF/paraffin embedding for IHC.
>I'm just wondering if any of you happen to have a good protocol for doing 
>so.  I know that for rat tissues one tends to use longer processing times..
>We just got a Citadel processing machine and I'd like to set up a program 
>for this.
>thanks a bunch in advance!

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610
406 994-6367
406 994-4303 (FAX)

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