Re: [Histonet] IHC background problems on chicken embryos
I would second Brian Chelack's suggestion to use a NON-biotinylated IHC
staining system with avian tissue. I use EnVision+/HRP exclusively (I stain
for a few avian viruses here). I would also suggest adding 2% normal
chicken serum per volume to the EnVision+/HRP reagent. It helps block
nonspecific binding of the EnVision to endogenous chicken Igs and serum
U of MN Vet Diag Lab
----- Original Message -----
From: "Katri Tuomala"
To: "HistoNet Server"
Sent: Monday, September 26, 2005 8:09 PM
Subject: [Histonet] IHC background problems on chicken embryos
> Hi Histonetters,
> A colleague of mine is trying to get VWF (Dakocytomation) and VEGF(R&D
> Systems) working on chicken tissue. The antibodies are performing well on
> human and rat tissue, but excessive background is the problem with the
> chicken. Would the blocking serums (normal goat and normal rabbit
> respectively) be the problem as well as the biotinylated secondary
> raised in goat and rabbit? My experience is strictly with human tissues,
> I am stumped. To my knowledge neither of these antibodies have been proven
> to work with chicken, so maybe that is the problem.
> Any advice from "chicken" people?
> Katri Tuomala
> Hamilton, Ontario, Canada
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