Would eosin Y do?  It fluoresces green.

Bryan Llewellyn


>> From: histonet-bounces@lists.utsouthwestern.edu on behalf of
>> Pixley, Sarah (pixleysk)
>> Sent: Tuesday, September 20, 2005 11:56 AM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Fluorescent cytoplasmic marker
>> 
>>  Dear Histonetters:
>> I need an antibody (or a stain) that will label all of the cytoplasm of
>> every cell. For example, a reliable (and hopefully cheap) antibody
>> against some common housekeeping protein that we can then localize with
>> a fluorescent secondary antibody. I want to be able to use the confocal
>> microscope to visualize the entire cytoplasm of every cell, because we
>> are asking questions about where the cells are relative to an unusual
>> substrate. We have tried staining for alpha and beta tubulin and the
>> filamentous staining is just not sufficient. We want to fill the
>> cytoplasm with a fluorescent marker. And we don't want to have to
>> transfect the cells or inject them. These are mouse cells.
>> Thanks,
>> Sarah Pixley
>> Univ. Cincinnati
>> 
>> 
>> 
>> 
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