Re: [Histonet] Bielschowsky Staining
Silver methods (if they are working properly)
stain the axons, not the myelin sheaths.
Unmyelinated and myelinated axons are shown. The
mechanism has not been thoroughly studied for
Bielschowsky-type methods, but in technically
simpler procedures such as Bodian's protargol
it is known that the substrate of staining is
a neurofilament protein within the axoplasm
(Gambetti et al. 1981 Science 213:1521-1522).
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
Emily Jane Wiesner-Camm wrote:
> Hello All.
> I have been staining some young rat brain sections with Bielschowsky
> stain, and I was wondering if anyone could tell me if the silver if
> deposited on only fully myelinated axons.
> Thank you in advance.
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