Re: [Histonet] 1% gelatin/40% etOH mounting solution

From:"John A. Kiernan"

Putting an adhesive on positively charged slides 
seems rather extravagant. The positive charge is
there to attract the predominantly negatively charged
carboxylate groups of the tissue's structural proteins.
Covering the surface of the glass with a layer of 
gelatin or chrome-gelatin (subbing) will occlude the
positive charges that you paid extra for.
for more information.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London,   Canada   N6A 5C1
Brandon Munk wrote:
> Histonetters,
> I am trying to get fixed tissue sections to stick onto slides better. I have
> tried subbed slides, plus slides (subbed and un-subbed) with only mediocre
> results. I was recently given a recipe for a 1% gelatin in 40% etOH mounting
> solution. This seems to work wonderfully on un-subbed plus slides, but I am
> having much difficulty in keeping the gelatin in solution long enough for it
> to be useful for mounting my sections. The recipe consists of heating
> distilled water (not over 60C) dissolving gelatin (enough for a 1% final
> concentration) and adding 80% etOH in two steps to the cooling gelatin
> solution, half soon after gelatin goes into solution and the other half
> sometime into the cooling of the solution. The recipe is vague since there
> is probably some voodoo that goes along with it. I have tried about 10
> different variations on this recipe, none work consistently. Does anyone
> have some tips, pointers or advice to help me out, or even another recipe
> that may work better. Thanks in advance!
> Brandon Munk
> Dept. Zoology and Physiology
> University of Wyoming
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