This question is a regular; I don't have a protocol as I am a Manager/
Cytologist but from memory fatty tissues are best cut thin, well fixed,
dehydrated and then put into a clearing agent/ degreasant. After the fat
has been 'removed' return to dyhydration, then 'clear' again and
impregnate with wax. You can use many degreasants but I used xylene, Mr.
Lillie 'waxes' eloquently about such matters.

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jennifer
N. Cresor
Sent: Tuesday, September 27, 2005 5:52 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] fatty tissue processing

Hello,
 
We are currently in the process of setting up a processor specifically
for
processing fatty tissues. I would really like to get people's protocols
on
processing fatty tissues that have worked well for them.  Thank you,
 
Jennifer
jcresor@icpath.com
 
 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mai