Try fixing the brains in 10% NBF instead.  We do rat brains all the time,
and the sections are beautiful.  Also, check your dehydration times in your
processing schedule.

Rebecca A. Davis, A.A.S., NYS LVT, HT (ASCP) 
Toxicology, Histopathology Lab 
Boehringer-Ingelheim Pharmaceuticals, Inc. 
rdavis4@rdg.boehringer-ingelheim.com 
203-798-5448 


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] 
Sent: Thursday, September 29, 2005 3:03 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] brain tissue

Dear Histonetters
I work with rat´s brains fixed in paraformaldehyde. I have problems with
paraffin inclusion and section. I always obtain brittle sections.
Help me
Thanks in advance

Dr. Hernán Aldana Marcos
haldana@unimoron.edu.ar
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