RE: [Histonet] antigen retrieval for IHC

From:"May Wei"

One of papers with Shi/Taylor is "Standardization of
Immunohistochemistry Based on Antigen Retrieval Technique for Routine
Formalin-fixed Tissue Sections" in Applied Immunohistochemistry 6(2):
89-96, 1998. I have the article. I also have the list of AR paper
references. If you like to receive it, I can send to you.

May Wei

-----Original Message-----
[] On Behalf Of John
Sent: Sunday, September 25, 2005 11:14 PM
To: Maria Mejia
Subject: Re: [Histonet] antigen retrieval for IHC

Maria, can you give chapter and verse for the Shi/Taylor and
Puchrler/Meloan papers? Most papers with Shi and Taylor among the
authors are about high temperature antigen retrieval (boiling water,
with pH optima for various antigens).
Holde Puchtler and Susan Meloan published many imortant papers about
staining ad histochemistry in the 1970s=1980s. Susan M was a histonetter
in the 1990s. 

Maria Mejia wrote:
> Hello,
> I believe it was sometime in June of this year that there was a 
> discussion on the histonet regarding "the simplest form" of reversing 
> the effects for formalin fixation on tissue was to wash the tissue 
> well (before) processing.
> Well, I just read the article "Antigen retrieval IHC: Past, Present, &

> Future by Shan-Rong Shi, richard J. Cote & Clive R. Taylor (can google

> this article). It's a very interesting! Anyway, the article has a 
> section titled non-heating AR method stating (this) same simple method

> by (Puchtler & Meloan). It also goes on to say that Elias JM (1990) 
> adopted this method routinely by storing deparaffinized in fresh 
> changes of 10% sucrose/PBS @ 4C overnight (before) IHC.
> My questions are, does anyone know or tried this method used by Elias?
> And, can anyone explain the mechanism of 10% sucrose/PBS play in this 
> AR method?
> Just curious!
> Maria Bartola Mejia
> Smith-Kettlewell Eye Research Institute San Francisco, CA 94115
> Email:
> Phone: (415)-345-2185
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