RE: [Histonet] RNAse decontamination in cryostats

From:"Favara, Cynthia (NIH/NIAID)"

Laurie,

I have not done this for a number of years but what I did was to use the
RNase-ZAP and then follow with a fresh - newly opened bottle of 100% ETOH -
remember the most common source of RNase is usually your hands so wear
gloves. Seemed to work well.

c

Cynthia Favara
NIAID/NIH/RML/LPVD
903 South 4th Street
Hamilton, MT 59840
406-363-9317

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-----Original Message-----
From: Patrick Laurie [mailto:plaurie@benaroyaresearch.org] 
Sent: Friday, September 23, 2005 12:29 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RNAse decontamination in cryostats

My question is similar to the current topic of discussion about
decontaminating cryostats, except that I need to remove any potential RNAse
activity.  I have been cleaning the disposable blade and the blade holder
with 70% alcohol, which hasn't been shown to eliminate the pernicious little
buggers, but the commercial products such as RNAse away (Ambion) etc, are
water based and would freeze on the blade/blade holder which would make me
suspicious of their effectiveness.  I am not able to continuously thaw the
cryostat and then use the products, as I would like to do daily.  I am
wondering if anyone has any ideas or potential products that would work.
Thanks

Patrick Laurie, HT (ASCP)
Neurogenomics Laboratory
Benaroya Research Institute
1201 9th Ave
Seattle, Wa  98101
(206) 341-0681


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