RE: [Histonet] Low temp Antigen Retrieval for Bone Sections
I haven't tried this method of antigen retrieval, but TEG buffer is
Tris-EGTA buffer. The usual formula (I have seen a couple of variations) is:
Distilled water 1,000 ml
Trisma base 1.21 gram
EGTA 0.19 gram
The usual target pH is between 8.95 and 9.0
EGTA is ethylene glycol bis(B-aminoethyl)-N,N,N',N' tetraacetic acid.
(The capital "B" in the above name represents "beta". My email program
doesn't support the symbol.)
> From: firstname.lastname@example.org on behalf of Kim
> Sent: Monday, September 26, 2005 11:34 AM
> To: Histonet
> Subject: [Histonet] Low temp Antigen Retrieval for Bone Sections
> Hello All,
> I will be performing some IHC on mouse femurs (about 12 or so different
> antibodies). In the past, I have had a lot of trouble keeping these
> sections on the slides during HIER. I did a quick search on the archives
> and there were lots of suggestions, but nothing definitive.
> I was reading an article about "low temp AR", overnight at 60C in TEG
> buffer, pH 9.0. Has anyone tried this method? Also - what does TEG buffer
> stand for?
> Any help would be greatly appreciated.
> Kim Merriam
> Cambridge, MA
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