RE: [Histonet] Fluorescent Illumination Systems: Suggestions please?
Melissa, if you haven't already, I would suggest you also post the question
on a microscopy listserve such as
Glenn Smith, P.Eng.
519.886.9013 x38 (office)
Biomedical Photometrics Inc/GeneFocus
Widefield Confocal Scanning Instruments and Software
Date: Mon, 26 Sep 2005 10:50:34 -0700
From: "Melissa Gonzalez"
Subject: [Histonet] Fluorescent Illumination Systems: Suggestions
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Sorry to re-post, but I only received one response to the following: I am
currently in the market to upgrade our existing HB0 100 lamp house, which is
very old, to several of new systems. Either the newest version of the HB0
100 from Zeiss, a mercury halide system called X-Cite from EXFO, or a xenon
based burner called Lambda LS from Sutter Instruments. They are all
comparable in price, but my main concern is increasing the brightness of my
signal and evenly illuminating the field of view. I mostly look at DAPI,
eGFP, FITC, and Alexa 594, which from spectral outputs, each one of these
systems appears to have a different weakness in one of the above. On a plus,
the Xcite rates their bulbs at 1500 hours, and the Lambda at 500-1000hrs.
Does anyone out there have any input/comments/experience with these systems?
(This would be hooked up to a Zeiss Axioplan) Please let me know if you have
any info, as I have been unsuccessful so far with demos and need to place an
Thanks a lot -I really appreciate it,
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