RE: [Histonet] Cutting fibers

From:"Monfils, Paul"

Hi Cindy,

In case the fibers don't section well, or don't adhere to the surrounding
tissue, cut some sections thicker than normal, which maximizes the chances
of the fibers remaining in the section.  Perhaps some sections at 5 microns,
some at 10 and a few at 20 to 30 microns.  Asbestos fibers for example tend
to shatter when the knife hits them.  But in a 20 micron section there will
often be some complete fibers, the knife having passed on each side of them
rather than through them.

> ----------
> From: on behalf of Cindy
> DuBois
> Sent: 	Tuesday, September 20, 2005 7:44 AM
> To: 	Histonet
> Subject: 	[Histonet] Cutting fibers
> I received a request from one of my pathologists.  He will be giving me a
> block of tissue with an unknown fiber embedded in the tissue.  He wants me
> to section the tissue retaining the fibers as much as possible so he can
> tell what the fibers are.
> Any suggestions would be greatly appreciated.
> Cindy DuBois
> Delta Pathology Assoc.
> Stockton, CA
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