can any one please tell me how to do antigen retrieval on soft tissue, what 
i do is i took cryosections on slide for human fetal brain  tissue age upto 
7 months (prenatal) sections are 40 micron thick, when i do HIER these 
sections are comming off the slides at some points, i use gelatin  subbed 
slides for this  please guide what to do in detail


thanking in advance

Arvind Singh Pundir

National Brain Research Centre
gurgaon, Haryana, INDIA
arvind@nbrc.res.in


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