[Histonet] Tissue fixation help

From:Angela McNabola

Good Morning,

Does anybody on the histonet have any experience with taking tissue that has
been snap frozen in minus 80, and then "thawed" out and formalin fixed and
paraffin embedded?

I won't get into the reasons why we have to do this, but does anyone have a
protocol (or just slowly thaw it out and fix it??).  How did your IHC turn out,
especially when using phosphorlyated antibodies?  Did you get good morphology of
your tissue?

thanks in advance!


Angela McNabola, MS HT(ASCP)SLS, QIHC
Bayer Healthcare
400 Morgan Lane
West Haven, CT 06516

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