[Histonet] Santa Cruz CD31 SC1506
|From:||"PALMER Jason (SVHM)" |
I queried Santa Cruz re this antibody in May this year, and below I have pasted part of their response to me - to summarize, a new batch is expected "by the end of September", which is just about upon us, obviously.
Bernard O'Brien Institute of Microsurgery
42 Fitzroy St, Fitzroy Victoria 3065
tel +61 3 9288 4018
fax +61 3 9416 0926
Date: Mon, 19 Sep 2005 11:15:26 -0500
From: "Jackie M O'Connor"
Subject: [Histonet] Santa Cruz CD31
Content-Type: text/plain; charset="us-ascii"
Does anyone know if Santa Cruz ever worked out the issue with their CD31
SC1506 antibody? Last I heard, their goat was sick.
Is it safe to order this antibody with a reasonable expectation that it
From: Julia Renstrom [mailto:email@example.com]
Sent: Fri 5/20/2005 3:34 AM
To: PALMER Jason (SVHM)
Subject: Re: problems with Santa Cruz sc-1506 antibody
Dear Dr. Palmer,
Thank you for your inquiry.
This antibody, sc-1506, is usually one of the strongest PECAM1 antibodies in the industry. However, we have seen some inconsistent staining of paraffin-embedded, formalin-fixed sections in recent lots of this antibody. However we had some problems with the goat that used to produce the IgG and immunized a new goat with the sc-1506 peptide immunogen. The new goat which is currently host to this antibody is producing IgG which is still performing in Western blot, but for some reason is not staining at the level we expect for paraffin-embedded sections.
Unfortunately, this is also the only appropriate product we can offer for your purposes -- it is the only mouse-reactive antibody which we recommend for paraffin-embedded sections. We have immunized yet another goat with the sc-1506 peptide immunogen and predict a release of this new IgG by the end of September, pending completion of our quality control testing.
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