Chris makes some very good suggestions.  We have found that tissues
subjected to HIER don't want to take up the green stain using the usual
staining procedures, but had not found the "magic bullet" to get them to
stain satisfactorally.  Other samples (no pretreatment or enzyme
pretreated) counterstain very well with methyl green.

Teri Johnson
Managing Director Histology Facility
Stowers Institute for Medical Research
1000 E. 50th St.
Kansas City, MO 64133

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