[Histonet] RE: Tissue fixation help

From:"C.M. van der Loos"


   Long  time  ago  we thawed a  frozen  tissue  sample directly in (room
   temp.)  NBF  (24-48 hs). Morphology was kept surprisingly well. Sorry,
   at that time phosphorylated antibodies were not even existant....

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   phone:  +31 20 5665631

   Date: Fri, 30 Sep 2005 08:25:24 -0400
   From: Angela McNabola 
   Subject: [Histonet] Tissue fixation help
   To: [1]Histonet@lists.utsouthwestern.edu
   Good Morning,
   Does  anybody  on  the histonet have any experience with taking tissue
   that has
   been snap frozen in minus 80, and then "thawed" out and formalin fixed
   paraffin embedded?
   I  won't  get into the reasons why we have to do this, but does anyone
   have a
   protocol  (or just slowly thaw it out and fix it??).  How did your IHC
   turn out,
   especially  when  using  phosphorlyated  antibodies?  Did you get good
   morphology of
   your tissue?
   thanks in advance!
   Angela McNabola, MS HT(ASCP)SLS, QIHC
   Bayer Healthcare
   400 Morgan Lane
   West Haven, CT 06516


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