[Histonet] Mayer's Haemalum reactive
I'd like to apologize if my question will sound too basic.
I have stained a tissue with Mayer's Haemalum reactive.
After I have put the specimen in water at a Ph a bit over 7, I would be expected a nuclear colour change from red "bordeaux" to blue.
But it didn't happen.
I fixed the specimen with Bouin's liquid and I clarified with Histoclear.
I wonder why.
Have you seen a behaviour of this kind?
Thanks in advance for your help,
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